Tamoxifen inducible crispr

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  • R01DA036865/DA/NIDA NIH HHS/United States
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  • Hayashi S et al (2002) Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation/inactivation in the mouse

    In conclusion, it can be stated that the use of CRISPR/Cpf1 genome editing system allowed us to generate transgenic rat ESCs expressing tamoxifen-inducible Cre recombinase

    K15-creERT2/VEGF flox) and would like to isolate the K15-creERT2 cells after tamoxifen Combinations of single gene KO strains or cKO strains with Cre-expressing or tamoxifen-responsive Cre–ERT2-expressing N

    Here, we develop an inducible CRISPR activation (CRISPRa) system by transgenic expression of doxycycline (Dox)-inducible dCas9-VPR in mouse embryonic stem cells (iVPR ESC)

    Tamoxifen is light sensitive, and should be made and stored in a light‐ blocking vessel (amber, or foil wrapped)

    CRISPR-Cas9-based genome editing enables the rapid genetic manipulation of any genomic locus without the need for gene We previously reported the efficient targeted introduction of transgenes into the genomic DNA of the common marmoset (Callithrix jacchus) using CRISPR-Cas9

    iMAP combines Cre-loxP and CRISPR-Cas9 technologies and utilizes a germline-transmitted transgene carrying a large array of individually floxed, tandemly

    B Summary of scratch assays in RD and Rh5 with tamoxifen-inducible CRISPR/Cas9-mediated HDAC6 targeting in RD and Rh5

    , tamoxifen-inducible Pax9-CreER mice, Axin2-mTurquoise2 mice) to investigate craniofacial development (Feng et al

    However, these forced administrations may be

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